Biochem/physiol Actions

Cell permeable: no

Reversible: no

Product does not compete with ATP.

Primary Targettrypsin-like serine proteases

General description

An alternative to DFP and PMSF. Specific irreversible inhibitor of trypsin-like serine proteases. Inhibits trypsin and thrombin in equimolar concentrations. Acts on C1r, C1s, Factor Xa and plasmin, requiring a 5- to 10-fold excess for complete irreversible inhibition. Inhibitory activity is approximately 1000-fold greater than that of PMSF. Stability is pH dependent: t_½ = 20 min at pH 6.0; 6 min at pH 7.0; 1 millisecond at pH 8.0.

Alternative to DFP and PMSF. An irreversible inhibitor of trypsin-like serine proteases. Inhibits trypsin and thrombin in equimolar concentration. Acts on C1r, C1s, Factor Xa, and plasmin, requiring a 5- to 10-fold excess for complete irreversible inhibition. Inhibitory activity is approximately 1000-fold greater than that of PMSF.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Cole, T.C., et al. 1989. Biochim. Biophys. Acta 990, 254.Laura, R., et al. 1980. Biochemistry19, 4859.

Packaging

5 mg in Plastic ampoule

Reconstitution

Unstable in solution; reconstitute just prior to use.

Warning

Toxicity: Standard Handling (A)