Analysis Note

Appearance (clearness): clear to turbidAppearance (color): yellowish-brownpH-value (25 °C): 7.0 - 7.4Growth promotion test in accordance with the current version of DIN EN ISO 11133.Inoculum on reference medium (Listeria monocytogenes ATCC 35152 (WDCM 00109)): ≤ 100Inoculum on reference medium (Listeria monocytogenes ATCC 13932 (WDCM 00021)): ≤ 100Inoculum on reference medium (Staphylococcus aureus ATCC 6538 (WDCM 00032)): ≥ 10000Inoculum on reference medium (Bacillus cereus ATCC 11778 (WDCM 00001)): ≥ 10000Growth (Listeria monocytogenes ATCC 35152 (WDCM 00109)): >10 colonies on ALOA medium, blue green with opaque haloGrowth (Listeria monocytogenes ATCC 13932 (WDCM 00021)): >10 colonies on ALOA medium, blue green with opaque haloGrowth (Staphylococcus aureus ATCC 6538 (WDCM 00032)): ﹤100 colonies on TSAGrowth (Bacillus cereus ATCC 11778 (WDCM 00001)): ﹤100 colonies on TSAMixed inoculum of Listeria and S.aureusAdditionaly monoculture of S.aureus and B.cereusConfirmation on ALOA medium resp. Tryptic soy agarIncubation: 20 - 26 hours at 30 ± 2°C, aerobicConfirmation on ALOA medium: 44 ± 4 hours at 37 ±1°CConfirmation on Tryptic soy agar: 24 ± 3 hours at 37 ± 1°C

Application

Granucult^® Modified UVM broth (Base) is suitable for the selective enrichment of Listeria in foods and beverages.

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

General description

Granucult^® Modified UVM broth (Base) is suitable for the selective enrichment of Listeria in the two-stage procedure. The combination of various peptones, extracts, salts, and buffer substances enables the good growth of Listeria. The selectivity is due to the antiproliferative substances nalidixic acid and acriflavine hydrochloride. The two-stage enrichment method has demonstrated its value, especially with sample materials (meat and meat products) that are characterized by a high level of accompanying flora.

Legal Information

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: 1.10824.5007

Packaging

500 g in Plastic bottle