N-Ethylmaleimide, BioUltra, >=99.0% (HPLC)

Code: 04259-5G D2-231

Application

N-Ethylmaleimide (NEM) has been used:to prepare NEM-modified myosin beadsto block unmodified free thiols in samplesas a supplement in IP lysis/wash buffe...


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€114.00 5G
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Application

N-Ethylmaleimide (NEM) has been used:to prepare NEM-modified myosin beadsto block unmodified free thiols in samplesas a supplement in IP lysis/wash buffer for in vitro SUMOylation assay

Reagent for the covalent modification of cysteine residues in proteins.

Biochem/physiol Actions

Sulfhydryl alkylating agent that inactivates NADP-dependent isocitrate dehydrogenase and many endonucleases.

Augments currents from native M-channels in sympathetic neurons and acts as an opener for KCNQ2, KCNQ4 and KCNQ5 channels.

absorptioncut-off at 382 nm in methanol at 1 M
anion tracessulfate (SO42-): ≤100 mg/kg, chloride (Cl-): ≤50 mg/kg
assay≥99.0% (HPLC)
bp210 °C (lit.)
cation tracesBi: ≤5 mg/kg, Na: ≤50 mg/kg, Mo: ≤5 mg/kg, Al: ≤5 mg/kg, K: ≤50 mg/kg, Li: ≤5 mg/kg, Cr: ≤5 mg/kg, Mn: ≤5 mg/kg, Sr: ≤5 mg/kg, Mg: ≤5 mg/kg, Ca: ≤10 mg/kg, Pb: ≤5 mg/kg, Cu: ≤5 mg/kg, Ba: ≤5 mg/kg, Fe: ≤5 mg/kg, Zn: ≤5 mg/kg, Ni: ≤5 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg
Gene Informationhuman ... SLC6A3(6531), SLC6A4(6532)
ign. residue≤0.05%
impuritiesinsoluble matter, passes filter test
InChI keyHDFGOPSGAURCEO-UHFFFAOYSA-N
InChI1S/C6H7NO2/c1-2-7-5(8)3-4-6(7)9/h3-4H,2H2,1H3
mp44-47 °C, 43-46 °C (lit.)
product lineBioUltra
Quality Level200
SMILES stringCCN1C(=O)C=CC1=O
solubilitymethanol: 1 M at 20 °C, clear, colorless
storage temp.2-8°C
Cas Number128-53-0
Hazard Class6.1
Un Number2928
Pack GroupII
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