Biochem/physiol Actions

kcat/Km = 1.7 x 105 M-1sec-1 for MMP-7 and 6.3 x 105 M_-¹ sec_-¹ for MMP-2

Cell permeable: no

Reversible: no

Primary TargetFluorogenic substrate for matrix metalloproteinases (MMPs)

Product does not compete with ATP.

General description

Extremely sensitive fluorogenic substrate for matrix metalloproteinases (MMPs). Suitable for assessment of total activity in crude preparations by continuous assays (k_cat/K_m = 1.7 x 10⁵ M^-1sec^-1 for MMP-7 and 6.3 x 10⁵ M^-1sec^-1 for MMP-2).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Knight, C.G., et al. 1992. FEBS Lett. 296, 263.

Physical form

Supplied as a trifluoroacetate salt.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Sequence

MCA-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH₂ ·TFA [Dpa = N-3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]

Warning

Toxicity: Standard Handling (A)