The FastGene® qFYR is a highest precision instrument for performing quantitative polymerase chain reaction (qPCR) experiments. qPCR is a well-established method for the sensitive detection and quantification of nucleic acids. During a measurement, the target DNA or RNA sequence is amplified, while a cycle-dependent increase of a fluorescent signal is detected in real-time. The FastGene® qFYR was developed to meet highest laboratory standards and deliver reliable performances for various real-time qPCR applications, including gene expression analysis, absolute and relative quantification, endpoint qualitative analysis, genotyping, gene mutation analysis, pathogen detection, GMO detection, protein stability screening, miRNA analysis, and melting curve analysis.

Due to the small distance between the scanning head and the plate, the FastGene® qFYR has an outstanding level of sensitivity and virtually no cross talk between the individual wells. 4 or 6 different colour channels are installed in the scanning head to be able to excite all commonly used qPCR dyes in a single plate scan (8 seconds).

Available in 2 different versions:

• 4+1 channels (FastGene® qFYR: MOL2001): 4 different colour channels and 1 second FAM channel
• Channel 1: FAM/SYBR green etc.
• Channel 2: VIC/JOE/HEX/TET etc.
• Channel 3: ROX/Texas red etc.
• Channel 4: Cy5 etc.
• Channel 5: FAM/SYBR green etc.
• 6 channels (FastGene® qFYR Plus: MOL2003): 6 different colour channels
• Channel 1: FAM/SYBR Green etc.
• Channel 2: VIC/JOE/HEX/TET etc.
• Channel 3: ROX/Texas Red etc.
• Channel 4: Cy5 etc.
• Channel 5: Cy5.5/Quasar 705/Alexa Fluor 680
• Channel 6: ATTO 42

Features:

• High sensitivity optical design: Has a unique, patented optical detection system. It combines a high quality PMT (photomultiplier tube) with a Fresnel lens that has a short focal length. The resulting short distance from detector to sample reduces signal loss and cross-talk between samples, and generally improves signal sensitivity.
• Superior thermal cycler performance: Delivers excellent temperature control, temperature precision (±0.2°C) and uniformity over the entire 96 well plate. The unique hollowed out thermal block design reduces its overall weight and ensures rapid heating and cooling rates (up to 6°C per second) for fast qPCR protocols. The system also uses state-of-the-art Peltier components for highest reaction quality and performance stability, providing reliable and consistent qPCR results.
• Powerful user software has it all: The included analysis software (FastGene® qFYR Analysis Studio) impresses with its particularly simple and operation. The menu is clearly structured and intuitively arranged. It responds to the needs of the user for different experimental setups, and personalised settings can be easily adjusted. Integrated analysis algorithms allow many steps, such as baseline subtraction or Cq value threshold calculation, to be performed automatically. Absolute and relative quantification of nucleic acids can also be automated. The software allows you to save predefined analysis settings for auto-exporting run data into a format of choice, including Excel, PDF, txt export format.
• Generate high quality data: The combination of innovative optics and a high-precision thermal block ensure ideal amplification conditions, making it ideal for various quantitative real-time PCR applications.
• Outstanding homogeneity: FastGene® qFYR shows great homogeneity across all wells of a 96 well plate.
• Powerful multiplexing: FastGene® qFYR Plus can discriminate up to 6 different targets in a single reaction well, helping you save time, conserve reagents, and reduce samples usage without sacrificing precision.
• Accurate quantification: The broad dynamic range of the FastGene® qFYR ensures a reliable and accurate quantification.
• 1.3-fold target discrimination: With the FastGene® qFYR, smaller concentration differences can be distinguished with high accuracy, underlining the high sensitivity of the device.
• Melt curve analysis: The Precision Melt Analysis Tool is a powerful method for probe-based allelic discrimination. This function is already included in the FastGene® qFYR analysis software and does not need to purchased additionally.
• Gradient qPCR for optimal annealing: The thermal gradient function of the FastGene® qFYR can be used to determine the optimal annealing temperature of a specific target.
• Standard plate format: 96 well block with gradient function