Harvard Apparatus QuikPrep Anion Exchange Chromatography Macro SpinColumn - Strong Anion Q

Code: QP744200 D2-0

QuikPrep® SpinColumns™ for anion exchange chromatography are filled with a positively charged resin to attract negatively charged molecules from samples. Matrices are available in either...


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€1,567.80 PK96
€1,928.39 inc. VAT

QuikPrep® SpinColumns™ for anion exchange chromatography are filled with a positively charged resin to attract negatively charged molecules from samples. Matrices are available in either strong or weak ionised states. This expresses the ability of the exchange matrix to maintain its charge with a change in pH and not the strength of the bond. Strong exchangers have functional groups that will always remain ionised, while the functional groups of the weak exchangers can be neutralised by changing the pH.

Ion exchange chromatography (IEX) is an effective method of sample purification and fractionation based on molecular charge. Samples are passed through spin columns containing a predetermined matrix. Target molecules are captured and held by ionic bonds to the insoluble stationary matrix while the remaining sample is allowed to flow through the column. The ionic bonds are then broken through the addition of an elution buffer which changes the pH and alters the ionic strength of the molecules being retained. The bound molecules are eluted in order of the least to most strongly bound allowing for the collection of samples in fractions for separate analysis.

Available Matrices (Packing Materials)
Functional GrouppH RangeIonic CapacityApplications
Strong Anion QQuaternary ammonium2 - 120.18 - 0.25mmol (sulfate ion)/mLHigh molecular weight protein separation
Weak Anion DEAEDiethylaminoethyl5 - 90.11 - 0.16mmol (Cl-)/mLProtein separation
Weak Anion PEILinear polyethylenimine4 - 80.4 - 0.5mmol (OH-)/gPeptide, protein, nucleic acid and oligonucleotide separation
Q = Q-Sepharose Fast Flow (quaternary amine sepharose)DEAE = Diethylaminoethlyl-celluloseLinear PEI = PolyWAX LP™ (silica based)
SpinColumn Types
Sample Volume, µLSample Capacity, µgElution Volume, µLIncludes
Ultra-Micro10 - 253 - 3028.52 x 2mL centrifuge tubes with top caps
Micro25 - 755 - 60502 x 2mL centrifuge tubes with top caps
Macro75 - 15030 - 3001432 x 2mL centrifuge tubes with top & bottom caps
96 Well Micro25 - 755 - 60502 x 96 well collection plate (1.1mL/well)
96 Well Macro25 - 15030 - 3001432 x 96 well collection plate (1.1mL/well)
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QP744150
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QP744151
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QP744204
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QP744410
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QP744411
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QP744700
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QP744701
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QP744704
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