Hisprep Fast Flow 16/10, Cytiva, 28-9365-51

Code: GE28-9365-51 D2-231

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Features and Benefits

Optimized for convenient s...


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€666.70 EACH
€820.04 inc. VAT
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Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Features and Benefits

Optimized for convenient scale-up purification of histidine-tagged proteins. Compatible with a wide range of reducing agents, detergents, denaturants, and other additives. Negligible leakage of Ni2+. High binding capacity, approx. 40 mg/mL medium. Convenient and time-saving 20 mL prepacked HiPrep™ format.

General description

HisPrep™ FF 16/10 is a ready-to-use column, prepacked with precharged Ni Sepharose® 6 Fast Flow. The column is an excellent choice for preparative, scale-up purification of histidine-tagged recombinant proteins.HisPrep™ FF 16/10 columns are prepacked with Ni Sepharose® 6 Fast Flow for scale-up purification of histidine-tagged proteins. Ni Sepharose® 6 Fast Flow consists of 90 ?m beads of highly cross-linked agarose, to which a chelating ligand has been immobilized. This chelating ligand is charged with Ni2+ ions, the first-choice metal ion for purifying most histidine-tagged proteins. The negligible leakage of Ni2+ ions ensures reliable capture of histidine-tagged proteins in repeated IMAC purifications.

Legal Information

HiPrep is a trademark of Cytiva

HisPrep is a trademark of Cytiva

Sepharose is a registered trademark of Cytiva

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)

average diameter90 µm
bed size16 mm × 100 mm
bed volume20 mL
capacity~40 mg binding capacity(histidine-tagged protein)
cleaning2-14(Ni2+-stripped medium.)
column I.D.16 mm
manufacturer/tradenameCytiva 28-9365-51
matrix6% cross-linked agarose
packagingpkg of 1 ea
parameter22 psi, 1.5 bar (22 psi) (Over the Packed Bed During Operation), ﹤10 mL/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)
particle size45-165 µm
shelf lifePlease be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
suitabilitysuitable for bioprocess medium
working range3-12(Ni2+-stripped medium.)
This product has met the following criteria to qualify for the following awards:



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