Air-Dryable™ Direct DNA qPCR and RNA/DNA qPCR Urine mixes are glycerol-free mixes that contain optimised excipients compatible with air and oven drying. The mixes have been designed for the development of quantitative PCR urine test assays for direct detection of DNA and RNA from human urine specimens without extraction. Can also be used wet and/or with purified nucleic acid.

Urine is an ideal clinical specimen because it is excreted in large quantities is non-invasive, and it can be self-sampled. Currently, urine specimens are used in the diagnosis and management of infectious diseases (including STDs), hormone and metabolic disorders, renal diseases, bladder cancer, urinary tract infections (UTIs), and for monitoring recreational drug use. However, urine contains substances such as urea and nucleases that can damage DNA or inhibit the PCR reaction.

Meridian’s new Air-Dryable™ Direct Urine molecular mixes are unique in that they enable the highly sensitive, very fast detection of target DNA or RNA directly from high concentrations of urine and are sensitive enough to detect arboviruses, such as Chikungunya virus. These mixes have been formulated specifically to overcome the inhibitors found in urine – no further optimisation is required aside from the addition of primers and probes. Furthermore, these mixes can be used in a wet format or dried down by oven or air drying to create ambient-temperature stable assays.

• Create your ultra-sensitive multiplex direct quantitative PCR urine test using crude urine samples
• Simplify qPCR urine testing, by removing the nucleic acid extraction step or inhibitor carryover during purification, shortening sample-to-result and reproducibility
• Designed for the direct detection of bacteria and cell-free nucleic acids at very low titers
• Can be used wet or it can be dried, for developing an ambient-temperate stable assay