Molecular diagnostic tests are progressively moving towards lyophilisation. There are several advantages for this, including room temperature shipping and storage, extended shelf-life and increased flexibility in sample volume. In order to be compatible with drying however, enzyme preparations must be glycerol-free and include specialised excipients that preserve the mixture as it is exposed to freezing, vacuum and dehydration.
Lyo-Ready 1-Step RT-qPCR Buffer is a glycerol-free qPCR mix containing RNase Inhibitor, reaction buffer, dNTP, MgCl2 and lyo-excipients. In order to produce room temperature lyophilised RT-qPCR reagents, Reverse Transcriptase, Taq polymerase, assay specific primers and probes are added to Lyo-Ready 1-Step RT-qPCR Buffer for subsequent lyophilisation.
- Used to create ultra-sensitive RT-qPCR assays
- Formulated with a specialised blend of lyo-excipients, compatible with lyophilisation into beads or cakes
- Minimises non-specific amplification for improved assay sensitivity and reliability
- High efficiency, giving excellent performance in multiplex RT-qPCR assays
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