Fast 1-Step RT-qPCR master mix contains Taq polymerase, reaction buffer, dNTP, MgCl2, stabilizers, PCR enhancers, and separate reverse transcriptase and RNase inhibitor. It is ideal for developing multiplex one-step qPCR diagnostic tests and is suited for high-throughput, automated platforms.
Fast 1-Step RT-qPCR Mix is a one-step RT-PCR master mix that has been designed with a highly optimised buffer chemistry, hot-start DNA polymerase, and reverse transcriptase, to give fast, efficient, reliable amplification of your low-copy RNA targets and subsequent highly sensitive, reproducible one-step RT-qPCR in a single tube. With high processivity and robustness, it delivers excellent results in both singleplex and multiplex qPCR assays and confers superior assay performance under fast thermal cycling conditions, allowing more samples to be run in a day with the highest confidence, ideal for high‑throughput assays.
- Create ultra-sensitive RT-qPCR assays to deliver reproducible results from low copy number RNA targets
- Robust formulation delivers consistent, reliable one-step RT-PCR performance in fast cycling conditions
- Minimises non-specific amplification for improved assay sensitivity and reliability
- High efficiency, giving excellent performance in multiplex qPCR assays
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