Application

α-Enolase human has been used in in vitro kinase activity assay.

Biochem/physiol Actions

α-Enolase catalyzes the dehydration of 2-phosphoglycerate to phosphoenolpyruvate. This protein plays a key role in anaerobic metabolism under hypoxic conditions and may act as a cell surface plasminogen receptor during tissue invasion. Abnormal expression of α-enolase is associated with tumor progression in some cases of breast and lung cancer. It also has been identified as an auto antigen associated with Hashimoto′s encephalopathy and severe asthma.

General description

α-Enolase, also known as Enolase 1, is one of three enolase isoenzymes and a glycolytic enzyme expressed in most tissues. It is predominantly expressed in the early stages of embryonic development. The enzyme consists of two subunits of 433 amino-acids each. Each subunit has a molar mass of 47kDa. The dimer is stabilized in the presence of magnesium.

α-Enolase is encoded by the gene mapped to human chromosome 1p36.3-p36.2.

Other Notes

MSILKIHARE IFDSRGNPTV EVDLFTSKGL FRAAVPSGAS TGIYEALELR DNDKTRYMGK GVSKAVEHIN KTIAPALVSK KLNVTEQEKI DKLMIEMDGT ENKSKFGANA ILGVSLAVCK AGAVEKGVPL YRHIADLAGN SEVILPVPAF NVINGGSHAG NKLAMQEFMI LPVGAANFRE AMRIGAEVYH NLKNVIKEKY GKDATNVGDE GGFAPNILEN KEGLELLKTA IGKAGYTDKV VIGMDVAASE FFRSGKYDLD FKSPDDPSRY ISPDQLADLY KSFIKDYPVV SIEDPFDQDD WGAWQKFTAS AGIQVVGDDL TVTNPKRIAK AVNEKSCNCL LLKVNQIGSV TESLQACKLA QANGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR SERLAKYNQL LRIEEELGSK AKFAGRNFRN PLAK

Physical form

1 mg/mL solution in 20 mM Tris-HCl (pH 7.5) containing 10% glycerol and 1 mM MgSO4.

Preparation Note

Centrifuge the vial prior to opening.