Analysis Note

Activity of human recombinant adiponectin is measured by its ability to inhibit proliferation of mouse myeloid cell line M1. The ED50 for the effect is typically 1-7 µg/ml.

Application

Adiponectin human has been used to study the effect of adiponectin on androstenedione secretion and oxidative stress in rodent model.

Biochem/physiol Actions

Adiponectin (also called Acrp30, AdipoQ) has antiatherosclerotic and anti-inflammatory properties. It plays a role in various physiological processes such as energy homeostasis and obesity. Plasma levels of adiponectin are reduced in obese humans, and decreased levels are associated with insulin resistance and hyperinsulinemia. It exhibits antidiabetic action by interacting with adiponectin receptors, AdipoR1 and AdipoR2. Low levels of adiponectin are also linked with hypertension and dyslipidemia. Mutation in the gene at position 276G is linked with risk for endometrial cancer.

General description

Adiponectin (also called Acrp30, AdipoQ) is an adipocyte specific secreted protein that circulates in the plasma. It is induced during adipocyte differentiation and its secretion is stimulated by insulin. The protein has a carboxyl-terminal globular domain and an amino-terminal collagen domain. The gene is mapped to human chromosome 3q27. Human adiponectin shares about 83% amino acid identity with that of mouse and about 90% with that of rat.

Physical form

Lyophilized from a 0.2 µm filtered solution in 25 mM Tris, 0.15 M NaCl, pH 7.4 containing 50 µg of bovine serum albumin (BSA) per 1 µg of adiponectin.

Reconstitution

Recontitute in PBS containing at least 0.1% BSA to a concentration of 0.1-1.0 mg/mL. This stock solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.

Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.