Transforming growth factor-beta 1 (TGF-β1) is a pleiotropic cytokine that regulates various cellular processes, including cell proliferation, growth, differentiation, motility, and apoptosis. It is an essential growth factor in many embryonic and induced pluripotent stem cell maintenance media, including the commonly used E8, StemPro, and mTeSR media. TGF-β1 also promotes the differentiation of various cell types such as fibroblasts, epithelial cells, and immune cells.
Human/bovine/porcine recombinant TGF-β1 PLUS™ protein is the first entirely animal origin-free recombinant human TGF-β1 protein for highly reproducible results and compatible with chemically-defined stem cell media.
TGF-β1 PLUS™ is a high purity 24 kDa dimer comprising optimised mature domain of TGF-β1 protein.
- High purity optimised human/bovine/porcine TGF-β1 protein (Uniprot: P01137)
- >98%, by SDS-PAGE quantitative densitometry
- 24 kDa (dimer)
- Expressed in E. coli
- Animal origin-free (AOF) and carrier protein-free.
- Manufactured in our Cambridge, UK laboratories
- Lyophilised from acetonitrile, TFA
- Resuspend in 10mM HCl at >100µg/mL (provided with protein and free of charge), prepare single use aliquots, add carrier protein if desired and store frozen at -20°C or -80°C