Application

Total protein kit, micro has been used to determine:total protein in the bacterial homogenate by Bradford methodtotal protein content by the Bradford microassay from the tissue extractsserum total proteinsoluble protein content by the Bradford method from leaves

Biochem/physiol Actions

Total protein kit, micro can be used to determine the concentration of proteins in solution.

General description

Protein determination is one of the most common operations performed in biochemical research. Total protein kit, micro provides a simple, rapid, and highly sensitive method requiring only 50 µL or less sample.

Principle

Brilliant blue G (Coomassie blue) reacts with protein in an acid-alcohol medium to form a blue-colored protein dye complex. The color, measured at 595 nm, is proportional to the protein concentration.

Suitability

Suitable for protein determination for samples of up to 0.5 mg/ml.

Testing Method

Colorimetric, Endpoint