Application

MITOCHONDRIAL PROTEIN IP KIT has been used to perform immunoprecipitation and co-immunoprecipitation (Co-IP) of mitochondrial proteins.

Biochem/physiol Actions

The mitochondrial protein immunoprecipitation (IP) kit also provides choices of detergents, N-dodecyl-β-D-maltoside, Triton X-100, and digitonin, to achieve different stringency conditions for protein-protein interaction studies. Triton X-100 is the most commonly used detergent, especially for membrane protein solubilization. The mitochondrial protein immunoprecipitation (IP) kit may be used for the following: optimized for compatibility with immunoprecipitation (IP and co-IP) and pull-down using tagged proteins gentle formulation for maintenance of stable mitochondrial complexes compatible with sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS PAGE), 2D gel, native gel, and mass spectrometry functional assays and enzymatic assays

General description

The mitochondrial protein immunoprecipitation (IP) kit contains a ready-to-use mitochondria protein IP buffer, optimized for immunoprecipitation (IP and co-IP) using mitochondria and mitochondrial extracts. The buffer is a gentle formulation, which maintains the stability of mitochondrial complexes.