Application

Affinity gel is suitable for the purification of histidine tagged proteins (His-tag proteins) in native and denaturing conditions.

Nickel Affinity Gel for the purification of His-tag^® proteins (histidine tagged proteins). HIS-Select^® High Flow (HF) brings the superior selectivity of HIS-Select technology to a highly cross-linked agarose for higher flow rates and mechanical stability under pressure. HIS-Select HF is designed for production scale purification and FPLC^™ applications. As with other HIS-Select products, the non-charged, hydrophilic linkage of the nickel-nitrilotriacetic acid (Ni-NTA) chelate group to the agarose ensures true one-step purification.

Features and Benefits

High selectivity for higher purity Unique non-charged hydrophilic linkage reduced non-specific binding Binding Capacity for histidine-tagged protein (His-tag protein) is greater than 15 mg/mL Highly cross-linked agarose matrix allows for purification under higher pressures Binding under denaturing or non-denaturing conditions One-step purification

General description

HIS-Select High Flow Nickel Affinity Gel is an immobilized metal-ion affinity chromatography (IMAC) gel that is designed to specifically bind His-tag proteins (histidine containing proteins) in chromatographic systems with pressures up to 200 psi and a maximum linear flow rate of 3,000 cm/hr.

Legal Information

HIS-Select is a registered trademark of Sigma-Aldrich Co. LLC

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

FPLC is a trademark of Cytiva

Physical form

1:1 suspension in a 30% ethanol solution

Preparation Note

Ethanol must be removed prior to use. Wash affinity gel in deionized water to remove the ethanol, then equilibrate with equilibration buffer that is provided.