Application
When performing small-scale affinity capture, such as immunoprecipitation, the affinity matrix is difficult to see in the microcentrifuge tubes. Accidental aspiration of the resin leads to quantitative variability in results. The EZview™ Red Affinity Gels greatly reduce the risk of pellet loss. EZview™ resins perform as well as conventional non-colored affinity gels, but allow the user to easily differentiate pellet from supernatant. This correlates to more accurate data because less protein is lost.
Sutitable for use with immunoprecipitation, western blotting,and enzyme assays.
Features and Benefits
Increased visibility - Red color reduces risk of incidental aspiration Improved recovery of target protein by reduced accidental loss Higher reproducibility - More consistent yields
General description
The EZ View Red Streptavidin Affinity gel is composed of streptavidin, covalently attached tocyanogen bromide-activated 4% agarose beads. It is designed to capture (pull-down) the biotinylated target molecules, such as proteins, peptides, antibodies, nucleic acids, lectins, receptors and ligands.
Legal Information
EZview is a trademark of Sigma-Aldrich Co. LLC
Packaging
1 mL in poly bottle
Physical form
1:1 (v/v) suspension in PBS containing 50% glycerol and 15 ppm Kathon
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