Analysis Note

Calculation of Total Antioxidant StatusThe antioxidant concentration of the Standard is used to calculate the antioxidant levels in the samples.1. Determine the ΔA for the samples, standard, and blank. ΔA = A - A_o2. Calculate the concentration of antioxidants in the sample using the formula below:﹤div class='Bio_doc_image'>Figure 2: Antioxidant Concentration Formula﹤/div>NOTE: If the antioxidant concentration is greater than 2.5 mM dilute the sample with 0.9% NaCl and re-assay. Typical antioxidant levels in normal human plasma are 1.30-1.77 mM.

Components

Substrate, Chromogen, Standard, Buffer, and a user protocol.

General description

Note: Spectrophotometer equipped with a temperature-controlled cuvette holder is strongly recommended for use with this kit.

A colorimetric assay kit for the measurement of the total antioxidant status. Each kit is suitable for performing a minimum of 50 assays.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Miller, N.J., et al. 1993. Clin. Science 84, 407.

Packaging

1 kit in Glass bottle

Preparation Note

Chromogen: Add 10 ml Buffer to each vial of Chromogen needed for the assay (10 tests per vial). After reconstitution, the Chromogen is stable for 2 days at 4°C or 8 h at room temperature.r> Substrate: Dilute each vial of Substrate needed for the assay by adding 7.5 ml Buffer. After dilution, the substrate is stable for 24 h at 4°C. NOTE: Some automated methods do not require substrate dilution.r> Standard: Add 1 ml distilled water to each vial of Standard needed for the assay. After reconstitution, the Standard is stable for 2 days at 4°C or 1 month at -20°C.

Principle

The Calbiochem™ Total Antioxidant Status Assay Kit is designed to assay antioxidant levels in serum or plasma samples. Additionally, the assay may be used to measure the antioxidant potential of (suitably solubilized) food and drug samples.

Storage and Stability

Upon arrival store the entire kit contents at 4°C. Note: The concentration of the standard is lot-specific. Please refer to the vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)