Components

Human Recombinant Calcineurin, Bovine Brain Calmodulin, RII Phosphopeptide Substrate, Assay Buffer, Phosphate Detection Reagent, Phosphate Calibration Standard, ½ volume 96-Well Plate, and a user protocol.

General description

A colorimetric assay kit for measuring calcineurin (PP2B) activity and for inhibitor screening. The assay is performed using RII Phosphopeptide Substrate (Cat. No. 207008).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Mondragon, A. et al. 1997. Biochemistry 36, 4934.Donella-Deana, A., et al. 1994. Eur. J. Biochem. 219, 109.Enz, A., et al. 1994. Anal. Biochem. 216, 147.Harder, K.W., et al. 1994. Biochem. J. 298, 395.Martin, B., et al. 1985. J. Biol. Chem. 260, 14932.

Packaging

1 kit in Glass bottle

Specifications

Assay time: 3.5 h

Storage and Stability

Store all components except the 96-Well Plate (room temperature) at -70°C for the highest stability. In particular, calcineurin must be handled with utmost care in order to retain its maximal enzymatic activity. Thaw it quickly in a water bath, maintained at room temperature, or by rubbing between fingers, then immediately store on an ice bath. Any remaining unused enzyme should be quickly refrozen by placing at -70°C. To minimize the number of freeze/thaw cycles, we recommend to aliquot the calcineurin into separate tubes and then store at -70°C.

Warning

Toxicity: Multiple Toxicity Values, refer to MSDS