Application

PTP Assay Kit 1 can be used to detect PTP-1B activity by dephosphorylation of the phosphopeptide (RRLIEDAEpYAARG) using malachite green detection, or by hydrolysis of pNPP.

Used to detect/quantify: PTP

Components

Tyrosine Phosphopeptide (RRLIEDAEpYAARG] (Cat.# 12-217)Malachite Green Solution A (Cat.# 20-105)Malachite Green Additive (Cat.# 20-104)Phosphate Standard (Cat.# 20-103)pNPP (p-Nitrophenyl Phosphate) (Cat.# 20-106)pNPP Tyr Assay Buffer (Cat.# 20-180)96-well microtiter plate

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Packaging

Kit capacity: 100 assays

1 kit in Kit capacity: 100 assays

Quality

routinely evaluated by malachite green and pNPP hydrolysis assays to detect PTP-1B (Catalog # 14-109 or Catalog # 14-358) activity by dephosphorylation of the phosphopeptide