Analysis Note

Low-end CV% Range: 0-19%|Low-end CV% Average: 6.5%

Application

Used to detect/quantify:IL-5

Research Sub CategoryCytokines & Cytokine Receptors

Research CategoryInflammation & Immunology

The sensitivity of Single Molecule Counting (SMC^®) technology gives the SMC^® Mouse IL-5 Discovery Immunoassay unparalleled performance in the quantification of IL-5 in mouse serum or EDTA plasma.

Components

Assay BufferMouse IL-5 Coating AntibodyAssay Plate10X PBSBlocking BufferStandard DiluentMouse IL-5 Detection AntibodyMouse IL-5 StandardSMC^® Mouse IL-5 Immunoassay Kit Instructions10X Wash BufferBuffer CElution Buffer B

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial

General description

The SMC^® Mouse IL-5 Discovery Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The SMC^® immunoassay system detects analytes using Single Molecule Counting (SMC^®) technology.

Interleukin-5 (IL-5) is a cytokine secreted mainly by T-cells and mast cells upon stimulation with antigens and allergens. It has pleiotropic effects toward B-cells and is involved in the production of immunoglobulins. More importantly, IL-5 acts in synergy with GM-CSF to govern the proliferation and maturation of eosinophils. A pronounced level of eosinophils is commonly associated with the development of allergic conditions such as asthma, eosinophilic esophagitis and hypereosinophilic syndrome. As such, IL-5 is highly regarded as a crucial mediator of the inflammatory pathway concerning allergic reactions. Current studies suggest that IL-5 blockade is a promising intervention for the treatment of allergic diseases.

Legal Information

SMC is a registered trademark of Singulex