Analysis Note

Low-end CV% Range: 1 - 11%|Low-end CV% Average: 6%

Application

Research Sub CategoryCytokines & Cytokine Receptors

Research CategoryInflammation & Immunology

The sensitivity of Single Molecule Counting , SMC^®, gives the Human GM-CSF Immunoassay Kit unparalleled performance in the quantification of GM-CSF in human EDTA plasma.

Components

GM-CSF Coated BeadsStandard DiluentGM-CSF Detection AntibodyAssay BufferGM-CSF StandardSMC^® Human GM-CSF Immunoassay Kit Instructions10X Wash BufferElution Buffer BBuffer D

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial

General description

Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) is a cytokine produced by T cells, B cells, macrophages, mast cells and fibroblasts in response to inflammation or infection. Its primary role is to stimulate granulocyte and monocyte production in the bone marrow. Once GM-CSF has bound to its receptor, the activated complex propagates a signaling cascade that increases macrophage counts in order to fight infection. Thus increased GM-CSF can be observed during episodes of infectious disease and in a spectrum of inflammatory disorders such as Crohn’;s disease. Conversely, a lack of GM-CSF resulting from autoantibody production has been linked to pulmonary alveolar proteinosis, leukemia, and neutropenia.

The SMC^® GM-CSF Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The SMC^® immunoassay system detects analytes using Single Molecule Counting (SMC^®) technology.

Legal Information

SMC is a registered trademark of Singulex