Analysis Note

Low-end CV% Range: 6 - 18%|Low-end CV% Average: 12%

Application

Research Sub CategoryCytokines & Cytokine Receptors

Research CategoryInflammation & Immunology

The sensitivity of Single Molecule Counting (SMC^®) technology gives the SMC^® IL-8 Immunoassay Kit unparalleled performance in the quantification of IL-8 in human EDTA plasma and serum.

Used to detect/quantify:IL-8

Components

Human IL-8 Coated BeadsStandard DiluentHuman IL-8 Detection AntibodyAssay BufferHuman IL-8 StandardSMC^® Human IL-8 Immunoassay Kit Instructions10X Wash BufferElution Buffer BBuffer D

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial

General description

The SMC^® Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The SMC^® immunoassay system detects analytes using Single Molecule Counting (SMC^®) technology.

Interleukin-8 (IL-8) is a CXC chemokine synthesized by many cell types, including macrophages, epithelial cells and endothelial cells in response to a pathogen or inflammatory stimulus. IL-8 functionendothelials as a chemotactic factor that attracts neutrophils, basophils, and T-cells. IL-8 is one of the major mediators of the inflammatory response and of innate immunity. Its function is to recruit neutrophils to phagocytose the foreign antigen. IL-8 is also a potent angiogenic factor that induces neutrophils to leave the bloodstream and enter into the surrounding tissue. IL-8 has been shown to have roles in the pathological angiogenesis that is associated with solid tumors, diabetic retinopathy and rheumatoid arthritis (RA).

Legal Information

SMC is a registered trademark of Singulex