Analysis Note

Low-end CV% Range: 1-11%|Low-end CV% Average: 7%

Application

The sensitivity of Single Molecule Counting (SMC^®) technology gives the SMC^® Human IL-21 Immunoassay Kit unparalleled performance in the quantification of IL-21 in human serum.

Research CategoryInflammation & Immunology

Components

Human IL-21 Coated BeadsStandard DiluentAssay BufferHuman IL-21 StandardHuman IL-21 Labeled Detection AntibodySMC^®IL-21 Immunoassay Kit Instructions10X Wash BufferElution Buffer BBuffer C

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial

General description

The SMC^® IL-21 Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The SMC^® immunoassay system detects analytes using Single Molecule Counting (SMC^®) technology.

Interleukin-21 (IL-21) is a cytokine with a potent regulatory effect on the immune system. Mainly produced by activated T-cells, IL-21 is responsible for the hematopoiesis of T-, B-, natural killer and dendritic cells. The production and differentiation of these effector cells may lead to an increased immunity against cancerous or virally infected cells. Thus, the role of IL-21 in the regulation of immuno-inflammatory responses has important implications for diseases such as multiple sclerosis, metastatic melanoma and metastatic renal cell carcinoma. Thus IL-21 will remain an attractive subject for drug development. Successful study outcomes will further establish IL-21 immunotherapy as an effective treatment for various cancers and inflammatory diseases.

Legal Information

SMC is a registered trademark of Singulex