Analysis Note

Colour (visual): yellow to orangeAppearance of substance (visual): powderIdentity (IR): passes testPurity (TLC (EtOAc/MeOH/H2O/DIPEA 60 : 30 : 10 : 3)): ≥ 98 %Assay (HPLC, area%): ≥ 99.0 % (a/a)For TLC-Systems see our General Catalog

Application

5-Carboxyfluorescein Novabiochem^®. CAS 76823-03-5, molar mass 376.32 g/mol.

General description

This high purity fluorescent dye (λ_ex494 nm, λ_em518 nm) is supplied as a single isomer, which ensures production of labeled products of defined chemical structure, as well as greatly simplifying purification and characterization. FAM should be introduced as the last step in the synthesis to avoid formation of side-products, or be protected by tritylation prior to acylation or hydrazine treatment [1].

The product number for this product was previously 01-63-0112.

To obtain a certificate of analysis (CoA) of a lot that begins with the letter “A”, please select the option in the right hand menu “Request a COA for Lot#s starting with A”.

Legal Information

Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: 01-63-0112