Application

ThermaStop^™-RT is suitable for gene expression quantitative PCR (qPCR) and next-generation sequencing (NGS) applications.

Features and Benefits

It eliminates the non-specific product formation Provides increased sensitivity and specificity Provides higher yield and pure cDNA synthesis for conventional RT-PCR It minimizes the primer-dimer formation It utilizes the same cycling conditions as conventional Taq polymerase Decreases the RNA secondary structure Stable at low temperatures Enzymes are inhibited at below 55°C Compatible with several RT-PCR enzymes Provides better multiplexing, efficient primer use Better quantitative accuracy and monoplex and multiplex amplification sensitivity

General description

ThermaStop^™-RT is a proprietary additive for One-Step, or Two-Step Reverse Transcriptase-PCR assays containing gene-specific primers. ThermaStop^™-RT interacts with the reverse transcriptase at low temperatures to reduce priming errors. ThermaStop^™-RT improves detection sensitivity and specific product yield by eliminating priming errors at low temperature. ThermaStop-RT acts as a Hot-start which binds and inhibits DNA polymerase activity. It also renatures the DNA polymerase upon cooling and activates its functions at lower temperatures or post-amplification.

Legal Information

ThermaStop is a trademark of ThermaGenix Inc.

ThermaGo is a trademark of ThermaGenix Inc.

Other Notes

RT-PCR, *Please note ThermaStop^™-RT should not be used in combination with ThermaStop^™ or ThermaGo^™.

Reconstitution

Dissolve in Tris pH 8.3 buffer