Aptamer Taq HS (glycerol-free) is a fast, high concentration, glycerol-free DNA polymerase containing a DNA aptamer that is designed to reversibly block the DNA polymerase and so work as a temperature-dependent hot start system. The glycerol-free formulation can be used to produce lyophilised or air-dried, ambient-temperature stable PCR or qPCR reagents and exhibit the same high performance before and after drying, offering greater flexibility for assay design and format.

Traditional hot start PCR methods that are antibody or chemically mediated require a long activation time at very high temperatures. In contrast, DNA aptamers disassociate from the enzyme (e.g. activate) at relatively lower temperatures (45°C), allowing for room temperature assay set-up, fast PCR reaction protocols and better assay specificity. Aptamer Taq HS (glycerol-free) is therefore suitable for difficult, multiplex PCR tests that require fast amplification whilst maintaining both high specificity and sensitivity.

• The DNA aptamer hot start suited to multiplex, high-throughput assays requiring high specificity
• Glycerol-Free DNA polymerase ideal for creating ambient temperature stable assays
• Provided as 50U/µL hot start Taq and enzyme dilution buffer
• Allows convenient room temperature reaction set-up