Features and Benefits
- Simple and reproducible: premixed with SYBR® Green I and fluorescein, just add primers and template, reducing errors in set-up
- Specific and sensitive: for detection of a wide range of template concentration, even from hard to obtain and low copy number samples
- Flexible: suitable for block-based instruments where fluorescein is required to allow calculation of dynamic well factors
- 2x master mix: premixed with SYBR® Green I and an internal reference for convenience
Instrument Compatibility: Bio-Rad iCycler®, iQTM5 and MyiQ™. However the kit can is also compatible with several instruments that do not require the use of fluorescein, such as the Qiagen Rotor-Gene™ 6000, the Bio-Rad CFX96 or the Roche LightCycler® 480.
SensiMix SYBR® & Fluorescein Kit contains fluorescein, making it specifically suitable for use on real-time instruments that measure fluorescein signal, such as the Bio-Rad iCycler®, iQ™5 and MyiQ™ platforms. Both SYBR® Green I and fluorescein are premixed for immediate use. With SYBR® Green I and fluorescein in their premixed and optimized concentrations, as few as 10 copies of a target gene can be detected.
Volume QT615-02: 250 x 50µl Reactions: 5 x 1.25ml QT615-05: 500 x 50µl Reactions: 10 x 1.25ml QT615-20: 2000 x 50µl Reactions: 40 x 1.25ml
Components: 2x Reaction mix consisting of: Heat-activated DNA polymerase, Ultra-pure dNTPs, MgCl2, Fluorescein SYBR® Green I; Plus separate vials of: 50mM MgCl2
Kit Size: The kit size is based on a 50µl final reaction volume.
Storage Conditions: 6 months at -20°C. Avoid exposure of the SYBR® Green I to light.
This product has met the following criteria: