There are a number of media available for the selective isolation of Neisseria gonorrhoeae of which this is one. Based on the medium developed by Thayer & Martin, it includes corn starch to absorb toxic metabolites and is buffered to maintain a neutral pH. The medium is made selective by the inclusion of LCAT (Lincomycin, Colistin, Amphotericin B & Trimethoprim). Lincomycin and colistin inhibit the growth of the majority of contaminating organisms likely to be present. Trimethoprim is included to reduce the spreading of Proteus spp and amphotericin B to inhibit yeasts and fungi. Yeast extract and glucose are also added as further enrichment along with laked horse blood to ensure an more luxuriant growth of Neisseria gonorrhoeae.
- Storage temperature: 2 to 8°C
- Appearance: firm gel
- Colour: cherry red
- Recommended incubation: 4 to 6% CO2 in air for 24 to 48 hours, best results are obtained if incubation takes place in high humidity (70 to 90%)
- pH: 7.2 ± 0.2
- Plate size: 90mm
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