Application

Suitable for use: As a tRNA standard As a substrate in tRNA synthatase assays As a substrate in other reactions involving tRNAs

Components

tRNA is provided as a lyophilized powder that is soluble in water at a concentration of 2 mg/ml.

General description

Transfer RNA is isolated from brewer’;s yeast. This transfer RNA is specific for transfer of the amino acid phenylalanine. The phenylalanine acceptor activity of 100% pure tRNA is 1,800 pmoles per A260 unit. Comparison of this number to lot-specific analysis may be used as an indication of product purity. Methods to further purify tRNA have been published.The assay for this product is based on a published method. The activity is measured using a crude preparation of phenylalanyl transfer ribonucleic acid synthetase. The phenylalanine specific tRNA is used as a substrate for the enzyme in the assay.This product is not suitable for use as carrier tRNA to aid in the ethanol precipitation of very small amounts of DNA. Product Nos. R 8508 and R 5636 are recommended for use as carrier tRNA for DNA purification and precipitation.

Preparation Note

Prepared by method of Wimmer, E., et al., Biochemistry, 7, 2623 (1968).