Analysis Note

Protein determined by biuret.

Application

α-glucosidase is used for the determination of α-amylase and the synthesis of various 1′-O-sucrose and 1-O-fructose esters. It was also used in the measurement of glycosidase inhibition.

For the determination of α-amylase and the synthesis of various 1′-O-sucrose and 1-O-fructose esters

Biochem/physiol Actions

α-glucosidase hydrolyzes carbohydrates by acting on 1,4-α linkages. Inhibition of α-glucosidase is a prominent target in the management of non-insulin-dependent diabetes mellitus.

Hydrolysis of terminal, non-reducing 1→4-linked D-glucose residues with release of D-glucose.

General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in starch hydrolysis research. For more information see the article in biofiles.

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Packaging

100 units in glass bottle

Sold on basis of p-nitrophenyl α-D-glucoside units.

1000 units in poly bottle

Unit Definition

One unit will liberate 1.0 µmole of D-glucose from p-nitrophenyl α-D-glucoside per min at pH 6.8 at 37 °C.