p-Nitrophenyl Phosphate Liquid Substrate System, liquid

Code: N7653-100ML D2-231

Application

p-Nitrophenyl Phosphate Liquid Substrate System has been used as a substrate to determine the activity of alkaline phosphatase in alkaline phosphatase as...


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List Price
€180.70 100ML
Your Price
€270.20 100ML
Save: €-89.50 (-50%)
€332.35 inc. VAT

Application

p-Nitrophenyl Phosphate Liquid Substrate System has been used as a substrate to determine the activity of alkaline phosphatase in alkaline phosphatase assay.

Substrate for alkaline phosphatase that yields a soluble yellow end product that may be read at 405 nm. The reaction may be stopped with 3N NaOH and read at 405 nm. Recommended for ELISA (microwell) procedures, not recommended for membrane applications.

Biochem/physiol Actions

4-Nitrophenyl phosphate disodium salt hexahydrate (pNPP) is a non-proteinaceous and non-specific substrate for determining protein tyrosine phosphatase activity. Hydrolysis of pNPP produces p-nitrophenol. It is a common chromogenic substrate for alkaline phosphatase.

Caution

Stable at least one year when stored below 0 °C.

General description

The p-Nitrophenyl Phosphate (pNPP) Liquid Substrate System combines p-nitrophenyl phosphate, buffer, and the required magnesium cations in a convenient, ready-to-use, single solution reagent. Producing a soluble end product, pNPP is the substrate of choice in alkaline phosphatase enzyme immunoassays.

Packaging

100 mL in glass bottle

Physical form

Ready-to-use.

formliquid
InChI keyVIYFPAMJCJLZKD-UHFFFAOYSA-L
InChI1S/C6H6NO6P.2Na/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;/h1-4H,(H2,10,11,12);;/q;2*+1/p-2
Quality Level200
shipped inwet ice
SMILES string[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1
storage temp.−20°C
Cas Number4264-83-9
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