Application

Developed for use as a soluble substrate for the detection of alkaline phosphatase activity in Enzyme Immunoassays (EIA). pNPP is the substrate of choice in high sensitivity alkaline phosphatase-based EIA assays.One pNPP tablet and one Tris buffer tablet are dissolved in deionized water and provide a ready-to-use substrate. Each Sigma FAST pNPP tablet set yields a solution containing 1.0 mg/ml pNPP, 0.2 M Tris buffer. EIA applications using pNPP may be read in timed assays or stopped with 3N NaOH for delayed readings. SIGMAFAST^™ require only the addition of distilled or deionized water to prepare an active substrate solution. Tablets are individually packaged in foil packets.

SIGMA FAST p-Nitrophenyl phosphate Tablets has been used: to quantify non-secreted alkaline phosphatase to monitor differentiation of osteoblast-like cells (SaoS-2)to stain murine preosteoblast MC3T3-E1 cells in alkaline phosphatase assayas a substrate for ExtrAvidin^® alkaline phosphatase to measure hemoglobin-bound α-synuclein using enzyme-linked immunosorbent assay (ELISA) as a substrate for alkaline phosphatase conjugated antibodies for detection in ELISA of abirin toxin

Legal Information

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

ExtrAvidin is a registered trademark of Sigma-Aldrich Co. LLC

Reconstitution

Each tablet set dissolved in 5 mL deionized water yields a ready-to-use buffered solution containing pNPP.