Application

Developed for use as a precipitating substrate for the detection of alkaline phosphatase activity in immunochemistry. Primary uses are in immunohistology, immunoblotting, or dot blotting. SIGMAFAST^™ Fast Red TR/Naphthol AS-MX tablets contain 0.6 mM levamisole to block endogenous alkaline phosphatase activity. SIGMAFAST^™ Fast Red TR/Naphthol AS-MX Tablets require no additional steps or buffers to prepare an active substrate solution.

SIGMAFAST Fast Red TR/Naphthol AS-MX Tablets has been used:in alkaline phosphatase staining in organ sectionsfor staining chondrogenic spheroids in immunohistochemistry studiesfor the detection of hybridization signals in in situ mRNA hybridization studies of human palatine tonsils sections

Legal Information

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

Reconstitution

Each tablet dissolved in 1 mL deionized water yields a ready-to-use buffered solution.