Application
Analytes: sCD137, Eotaxin/CCL11, sFasL, FGF-2, Fractalkine/CX3CL1, Granzyme A, Granzyme B, IL-1α, IL-2, IL-4, IL-6, IL-16, IL-17A, IL-17E/IL-25, IL-21, IL-22, IL-23, IL-28A, IL-31, IL-33, IP-10/CXCL10, MIP-3α/CCL20, Perforin, RANTES/CCL5, TNFβ/LTA Recommended Sample type: Serum, plasma and cell culture samples Recommended Sample dilution: 1:2 diluted plasma or serum NOTE: RANTES/CCL5 is for tissue culture samples only and cannot be combined with other analytes in this panel when measuring serum or plasma due to different dilution needs Assay Run Time: Overnight or one day. For best results, an overnight incubation is recommended Research Category: Inflammation & Immunology
Components
Non-Human Primate Cytokine Panel 2 Standard, 1 vial, lyophilizedNon-Human Primate Cytokine Panel 2 Quality Control 1, 1 vial, lyophilizedNon-Human Primate Cytokine Panel 2 Quality Control 2, 1 vial, lyophilizedSerum Matrix (0.08% Sodium Azide), 1 bottle, lyophilizedSample Plate, 96 well format, 1 plate2 Foil plate sealersAssay Buffer, 2 bottles, 30 mLBead Diluent, 1 bottle, 3.5 mLWash Buffer, 10X (0.05% Proclin), 2 bottlesl, 30 mLNon-Human Primate Cytokine Panel 2 Detection Antibodies, 1 bottle, 3.2 mLStreptavidin-Phycoerythrin, 1 bottle, 3.2 mLPremixed 24-plex Beads, 1 bottle, 3.5 mLRANTES beads, 1 vial
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Cytokines, immunomodulatory polypeptides that play key roles in both adaptive and innate immune responses, are one of the regulatory mechanisms of the immune system. Acting at the recognition, activation or effector phases of an immune response, cytokines modulate the development and functional activities of the subtypes of T cells, B cells and myeloid cells. Since chronic inflammation and aberrant immune response contribute to many disease states, research involving cytokines and chemokines plays a significant role in achieving a deeper understanding of the etiology and progression of diseases such as infectious disease, autoimmune and chronic inflammatory disease, cardiovascular disease, metabolic syndrome, neurological disorders and cancer. The MILLIPLEX® Non-Human Primate Premixed Cytokine Panel II is a 25 plex kit to be used for the simultaneous quantification of the following analytes in monkey serum, plasma, tissue/cell lysate and culture supernatant samples: sCD137, Eotaxin, sFASL, FGF-2, Fractalkine, Granzyme A, Granzyme B, IL-1α, IL-2, IL-4, IL-6, IL-16, IL-17A, IL-17E/IL-25, IL-21, IL-22, IL-23, IL-28A, IL-31, IL-33, IP-10, MIP-3α, Perforin, RANTES and TNFβ. The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume. Panel Type: Cytokines/Chemokines
Legal Information
xMAP is a registered trademark of Luminex Corp
Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
Other Notes
Sensitivity: Refer to kit protocol for sensitivities of individual biomarkers.
Specificity
Cross ReactivtyThis multiplexed assay panel has been tested with samples from multiple non-human primate species. Please see protocol for details.
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Storage and Stability
Recommended storage for kit components is 2 - 8°C.