Thermo Scientific™ SurePac™ Oligo RP MDi™ HPLC columns deliver high-resolution, reproducible separations for therapeutic oligonucleotides and nucleic acids across R&D and QC workflows. Built on monodisperse, supermacroporous polymeric packing with bio-inert hardware, they provide stable performance even under elevated pH and temperature conditions, resolving truncation impurities while supporting large oligos and intact nucleic acids for scalable LC-MS and LC-UV analysis.
- Purpose-built for oligonucleotide RP separations
- Optimized for DNA and RNA oligonucleotides, including modified sequences, delivering consistent retention and selectivity across short and long oligos
- Bioinert, metal-free fluid path
- Reduces metal/oligonucleotide interactions to improve recovery, minimize adsorption, and deliver cleaner peak shapes for sensitive analyses
- Optimized for ion-pair RP LC-MS
- Fully compatible with common ion-pairing reagents such as HFIP/TEA, enabling sensitive, reproducible LC-MS detection
- High recovery of long and modified oligonucleotides
- High resolution power for oligonucleotides spanning over a wide size range from small to (ultra) large
- Supports efficient analysis of long, hydrophobic, and chemically complex oligos without compromising signal or resolution
- Robust stationary phase for demanding methods
- Maintains stable performance under high organic content and elevated temperatures, supporting fast gradients and long column lifetimes
- Delivers sharp, symmetrical peaks and reliable separation of closely related species, including impurities and sequence variants
- Suitable for method development, impurity profiling, and QC, reducing the need for multiple column chemistries
- Compatible with UHPLC and HPLC systems
- Designed for seamless integration into modern LC platforms and existing laboratory setups
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