Application

Research CategoryStem Cell Research

Immunocytochemistry: Recommended working dilution is 1:500.

Detect SRF [Serum Response Factor] using this Anti-SRF [Serum Response Factor] Antibody, clone 1E1 validated for use in WB & IC.

Research Sub CategoryMesenchymal Stem CellsNeural Stem Cells

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Serum response factor (SRF) is a nuclear transcription factor that binds to the serum response element (SRE), and is essential for embryonic development of the mesoderm, as well as for the maintenance of adult muscle cells and neurons. As this protein is involved in the downstream regulation of many immediate-early genes, it participates in a variety of cell functions, including cell cycle, apoptosis, cell growth, and cell differentiation. SRF is most commonly activated by serum, LPA, LPS, TPA, growth factors, including TNFalpha, as well as antioxidants and UV light.

Immunogen

Synthetic human SRF peptide, a.a. 406-508.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl, and 0.05% sodium azide.

Quality

(Based upon representative data from a previous lot).

Western Blotting: Recommended working dilution is 0.5 µg/mL.

Specificity

This monoclonal antibody recognizes SRF.

A weak interaction with mouse has also been observed. Reactivity with other species has not been tested.

Storage and Stability

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months. Avoid repeat freeze/thaw cycles.

Target description

~52 kDa