Analysis Note

ControlMouse embryonic Stem Cells

Application

Research CategoryStem Cell Research

Research Sub CategoryPluripotent & Early Differentiation

Anti-SSEA-1 Antibody, clone MC-480, PE conjugate is an antibody against SSEA-1 for use in IH, IP, IF, FC.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

SSEA-1 is expressed on the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine & human germ cells (EG). No immunoreactivity is evident with undifferentiated human EC and ES cells. Expression of SSEA-1 is down regulated following differentiation of murine EC and ES cells. In contrast, the differentiation of human EC and ES cells is characterized by an increase in SSEA-1 expression.

Immunogen

Epitope: MC-480

F9 tetracarcinoma stem cells (X-irradiated).

Other Notes

This antibody reacts with the Stage-specific embryonic antigen-1 (SSEA-1) that is expressed upon the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine & human germ cells (EG).

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Ammonium Sulfate Precipitation

Purified mouse monoclonal IgM conjugated to PE fluorochrome in PBS with 0.1% sodium azide and 15 mg/mL BSA.

Quality

Evaluated by Flow Cytometry with mouse embryonic stem cells.

Specificity

This antibody reacts with the Stage-specific embryonic antigen-1 (SSEA-1) that is expressed upon the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine & human germ cells (EG).

Storage and Stability

Maintain refrigerated at 2-8oC in undiluted aliquots for up to 6 months from date of receipt. Protect from light.

Target description

220 kDa Calculated