Lipopolysaccharides from Escherichia coli 0127:B8, BioXtra, suitable for cell culture, gamma-irradiated

Application

Lipopolysaccharides from Escherichia coli 0127:B8 has been used:to study the LPS-induced macrophage survival in mice to elicit the secretion of cytokines by cattle PBMC to study apoptosis of highly susceptible macrophage cells in a LPS-induced proinflammatory environmentto perform autophagy assay in BV2 and BV2-LC3 cellsfor stimulation of human monocytes, macrophages and mouse microglia cellsto induce pentraxin-3 expression in 293T cells

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).

Biochem/physiol Actions

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4). Being an immunogenic antigen, it enhances immune responses to soluble antigens.

Reconstitution

Lipopolysaccharides are supplied as lyophilized, γ-irradiated powders. To reconstitute, add 1 ml sterile balanced salt solution or tissue culture medium to the vial (1 mg) and gently swirl until the powder dissolves. Reconstituted product may be further diluted to desired working concentrations using sterile balanced salt solution or tissue culture medium.