Application

GPDH (glyceraldhyde-3-phoshate dehydrogenase) activity assay kit has been used to measure the serum and hepatic concentrations of GPDH in rabbit.

General description

Glycerol 3-phosphate dehydrogenase (GPDH; EC 1.1.1.8) catalyzes the reversible conversion of dihydroxyacetone phosphate and NADH to glycerol-3-phosphate and NAD⁺. It forms a glycerol phosphate shuttle and facilitates the transfer of reduced equivalents across the mitochondria to the cytosol. GPDH is important for both carbohydrate and lipid metabolism. Mutations in GPDH gene result in transient infantile hypertriglyceridemia.

Principle

The Glycerol 3-Phosphate Dehydrogenase Activity Colorimetric Assay Kit provides a simple and sensitive procedure for measuring GPDH activity in a variety of tissues and cells. GPDH activity is determined by measuring a colorimetric product with absorbance at 450 nm (A450) proportional to the enzymatic activity present. One unit of GPDH is the amount of enzyme required to generate 1.0 µmole of NADH per minute at pH 8 at 37 °C.

Suitability

Suitable for the detection of Glycerol-3-Phosphate Dehydrogenase Activity in tissue and cell culture samples.