Application

NAD/NADH Quantitation Kit has been used to quantify NAD⁺/NADH influenced by SIRT1 (sirtuin 1) activity (associated with ischemia-reperfusion injury) in the presence of trimetazidine.

General description

Nicotinamide adenine dinucleotide (NAD) is an enzymatic cofactor involved in many redox reactions. NAD functions as an electron carrier, cycling between the oxidized (NAD) and reduced (NADH) forms. In addition to its role in redox reactions, NAD plays critical roles in ADP (adenosine diphosphate)-ribosylation reactions and as a substrate for sirtuins.

Principle

The NAD/NADH Quantification Kit provides a convenient tool for sensitive detection of the intracellular nucleotides, NAD (nicotinamide adenine dinucleotide) and NADH, and their ratio without the requirement to purify NAD/NADH from samples. This assay is specific for NAD and NADH, and does not detect NADP or NADPH. NADt (NAD and NADH) or NADH are quantified in a colorimetric assay (450 nm).

Suitability

Suitable for the detection of NADH and NAD in animal tissues (liver, kidney etc.), cell culture (adherent or suspension cells), serum or urine.