TWEEN 20, viscosity 250-450 mPa.s (25 C)

Code: 93773-1KG D2-231

Application

Non-ionic detergent

TWEEN 20 is widely used in several biochemical applications. It has been used:As an emulsifying agent for the preparation of stable oil-...


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Your Price
€149.00 1KG
€183.27 inc. VAT

Application

Non-ionic detergent

TWEEN 20 is widely used in several biochemical applications. It has been used:As an emulsifying agent for the preparation of stable oil-in-water emulsionsIn pre-extraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins)As a blocking agent for membrane based immunoassays at a typical concentration of 0.05% - 1%for lysing mammalian cells at a concentration of 0.005 to 0.5%For rehydrating tissue before in situ hybridization

Caution

may get turbid on storage, heat to clear

General description

Tween®20 is a polyoxyethylene sorbitol ester that belongs to the polysorbate family. It is a nonionic detergent having a molecular weight of 1,225 daltons, assuming 20 ethylene oxide units, 1 sorbitol, and 1 lauric acid as the primary fatty acid. The ethylene oxide subunits are responsible for the hydrophilic nature of the surfactant, while the hydrocarbon chains provide the hydrophobic environment. Sorbitol forms the backbone ring to which the ethylene oxide polymers are attached.

Legal Information

TWEEN is a registered trademark of Croda International PLC

density1.11 g/mL at 20 °C, 1.095 g/mL at 25 °C (lit.)
descriptionnon-ionic
formviscous liquid
HLB value16.7±1.0
InChI keyHMFKFHLTUCJZJO-UHFFFAOYSA-N
InChI1S/C26H50O10/c1-2-3-4-5-6-7-8-9-10-11-24(30)34-19-18-31-20-22(32-15-12-27)26-25(35-17-14-29)23(21-36-26)33-16-13-28/h22-23,25-29H,2-21H2,1H3
mol wt~1228
Quality Level200
refractive indexn20/D 1.471, n20/D 1.468 (lit.)
technique(s)DNA extraction: suitable, RNA extraction: suitable, protein purification: suitable, cell culture | mammalian: suitable
viscosity250-450 mPa.s(25 °C)
Cas Number9005-64-5
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