Anti-ULBP-4/RAET1E,cl 6C10 ZooMAb�Rbmono

Application

Anti-ULBP-4/RAET1E, clone 6C10 ZooMAb, Cat. No. ZRB1034, is a recombinant Rabbit monoclonal antibody that specifically targets Retinoic acid early transcript 1E and is tested for use in Affinity Binding Assay, Immunocytochemistry, and Western Blotting.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected ULBP-4/RAET1E in ULBP-4 cell lysate.Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected ULBP-4/RAET1E in HT-29 cells.Affinity Binding Assay: A representative lot of this antibody bound human recombinant ULBP-4 protein with a KD of 4.5 x 10-8 in an affinity binding assay.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.Learn more about ZooMAb here.

Immunogen

His-tagged recombinant fragment corresponding to 195 amino acids from the extracellular domain of human Retinoic acid early transcript 1E (RAET1E).

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

0.3 mg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Specificity

Clone 6C10 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects human Retinoic acid early transcript 1E. It targets an epitope within the extracellular domain.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Target description

Retinoic acid early transcript 1E (UniProt: Q8TD07; also known as Lymphocyte effector toxicity activation ligand, NKG2D ligand 4, N2DL-4, NKG2DL4, RAE-1-like transcript 4, UL16-binding protein 4, ULBP-4) is encoded by the RAET1E (also known as LETAL, N2DL4, ULBP4, UNQ1867/PRO4303) gene (Gene ID: 135250) in human. ULBP-4 is a single-pass type I membrane protein that is synthesized with a signal peptide (aa 1-30), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 31-225), a transmembrane domain (aa 226-248), and a cytoplasmic domain (aa 249-263). It is predominantly expressed in the skin, but also expressed in testis and trachea. Its levels are upregulated in tumor cells of different origins and its expression progressively decreased after treatment of tumor cells with retinoic acid. ULBP-4 also serves as a functional ligand for NKG2D. ULBP-4 is reported to bind and co-stimulate NKG2D-expressing effector cells including NK cells, NKT cells, CD8+ T cells. ULBP-4 has a MHC class I alpha-1 like (aa 31-116) and MHC class I alpha-2 like (117-207) regions that down-regulate the cell surface expression of Killer Cell Lectin Like Receptor K1 (KLRK1). Six different isoforms of ULBP-4 have been described that are produced by alternative splicing. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Chalupny, NJ., (2003). Biochem. Biophys. Res. Commun. 305(1); 129-135).