ANTI-GLUT-1; CT; CLONE 16D21; ZOOMAB RA

Code: ZRB071401-25UL D2-231

Application

Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected GLUT-1 in human placenta tissue sections.Immunofluorescence Analysis...


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Application

Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected GLUT-1 in human placenta tissue sections.Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected GLUT-1 in human placenta frozen sections.Immunocytochemistry Analysis: A 1:100-250 dilution from a representative lot detected GLUT-1 in NIH3T3 and HepG2 cells.

Anti-GLUT-1, CT, clone 16D21 ZooMAb, Cat. No. ZRB071401, is arecombinant Rabbit Monoclonal Antibody that specifically targets GLUT1 and has been tested for use in Immunocytochemistry, Immunofluorescence., Immunohistochemistry, and Western Blotting,

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.Learn more about ZooMAb here.

Immunogen

KLH-conjugated linear peptide corresponding to 12 amino acids from the C-terminal region of human Solute carrier family 2, facilitated glucose transporter member 1 (GLUT-1).

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Specificity

Clone 16D21 is a a ZooMAb rabbit recombinant monoclonal antibody that specifically detects human and murine GLUT-1. It targets an epitope with in 12 amino acids from the C-terminal region.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Target description

Solute carrier family 2, facilitated glucose transporter member 1 (UniProt: P11166; also known as Glucose transporter type 1, erythrocyte/brain, GLUT-1, HepG2 glucose transporter) is encoded by the SLC2A1 (also known as GLUT1) gene (Gene ID: 6513) in human. Glucose transporters are a family of integral membrane proteins that facilitative glucose uptake by cells. Seven different glucose transport proteins have been described that are designated as GLUT-1 to 7. GLUT-1 is a highly conserved; ubiquitously distributed, multi-pass membrane protein that is responsible for constitutive glucose uptake. It displays very broad substrate specificity and can transport a wide range of aldoses including both pentoses and hexoses. It is the predominant glucose transporter in embryonic and fetal tissues. In many organs, GLUT-1 is concentrated in endothelial cells of blood-tissue barriers. Hence, it has a specialized role to shuttle glucose between blood and organs that have limited access to small solutes via passive diffusion. It is also abundant in the mammalian erythrocyte membrane where it can rapidly equilibrate glucose between the cytoplasm of the erythrocyte and the blood plasma. GLUT-1 levels are reported to be frequently upregulated during tumorigenesis. Mutations in SLC2A1 gene are linked to GLUT-1 deficiency syndrome 1 and 2 that are characterized by infantile-onset epileptic encephalopathy, delayed development, microcephaly, and motor incoordination, and paroxysmal exercise-induced dyskinesia. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

antibody formpurified antibody
antibody product typeprimary antibodies
biological sourcerabbit (recombinant)
clonerecombinant monoclonal, 16D21, monoclonal
conjugateunconjugated
enhanced validationrecombinant expressionLearn more about Antibody Enhanced Validation
formlyophilized
Gene Informationhuman ... SLC2A1;GLUT1(6513)
greener alternative categoryAligned
greener alternative product characteristicsWaste PreventionDesigning Safer ChemicalsDesign for Energy EfficiencyLearn more about the Principles of Green Chemistry.
isotypeIgG
mol wtcalculated mol wt 54.08 kDa, observed mol wt ~52 kDa
packagingantibody small pack of 25 µL
product lineZooMAb® learn more
recombinantexpressed in HEK 293 cells
shipped inambient
species reactivity (predicted by homology)bovine, rat
species reactivityhuman, mouse
storage temp.2-8°C
technique(s)immunocytochemistry: 1:100-1:250, immunofluorescence: suitable using 1:100, western blot: suitable using 1:1,000, immunohistochemistry: 1:100-1:250
UniProt accession no.P11166
This product has met the following criteria to qualify for the following awards:



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