Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.Chromatin immunoprecipitation (1 paper)

Anti-Twist1 antibody produced in rabbit has been used in:immunohistochemistryimmunofluorescenceimmunoblottingchromatin immunoprecipitation (ChIP)

Anti-Twist1 antibody is suitable for use in detecting Twist1 via in immunochemical applications such as immunoblotting (~26 kDa) and immunofluorescence.Immunoblotting: a working antibody concentration of 0.5-1 mg/mL is recommended using extracts of human 293T cells expressing recombinant human Twist1 and a chemiluminescent detection reagent.

Biochem/physiol Actions

Twist is required for mesoderm formation. Twist 1 controls embryonic morphogenesis. It also plays a key role in metastasis by inducing an epithelial-mesenchymal transition (EMT). During an EMT, Twist stimulates the expression of mesenchymal markers, such as fibronectin and N-cadherin. In addition, it also blocks E-cadherin expression inhibiting cell-cell adhesion and therefore inducing infiltrative tumor growth in several human carcinomas. Twist1 is required in head mesenchyme for cranial neural tube morphogenesis in mice. Twist1 is involved in osteoblast differentiation and maturation. Increased expression of Twist 1 in breast cancer cells induce angiogenesis and leads to chromosomal instability. Mutations in this gene have been found in patients with Saethre-Chotzen syndrome, an autosomal dominant defect characterized by minor skull and limb anomalies.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Twist is encoded by the gene mapped to human chromosome 7p21.1. The encoded protein is a basic helix-loop-helix (bHLH) transcription factor. Twist 1 belongs to the Twist gene family and is mainly expressed in the suture mesenchyme.

Immunogen

synthetic peptide corresponding to amino acids 12-27 of human Twist1 with a C-terminal added cysteine, conjugated to KLH. The corresponding peptide sequence is identical in both rat and mouse.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Specificity

Anti-Twist1 antibody recognizes human Twist1. Detection of the Twist1 band by immunoblotting is specifically inhibited with the immunizing peptide.