Analysis Note
Positive ControlLNCaP cells
Negative Control293T cells
Application
ELISA (see comments)Immunoblotting (1-5 g/ml)
General description
Purified mouse monoclonal antibody. Recognizes the ~26 kDa EDN3 protein.
Anti-EDN3, mouse monoclonal, clone 2A6-2A4, recognizes the ~26 kDa EDN3 protein in LNCaP cells. it is validated for use in ELISA and Western blotting.
Recognizes the ~26 kDa EDN3 protein in LNCaP cells.
Immunogen
Full-length, recombinant human EDN3 (aa 1-239), expressed as a GST fusion protein
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Other Notes
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Physical form
In PBS, pH 7.2.
Reconstitution
Following initial thaw, aliquot and freeze (-20°C).
Warning
Toxicity: Regulatory Review (Z)
This product has met the following criteria to qualify for the following awards: