MONOCLONAL ANTI-PROLIFERATING PROTEIN K

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.Immunocytochemistry (1 paper)

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.Immunocytochemistry.Monoclonal Anti-Proliferating Cell Protein Ki-67 antibody produced in mouse has also been used for immunohistochemistry.

Biochem/physiol Actions

Marker of proliferation Ki-67 (MKI67), which is a part of the mitotic chromosome periphery, acts as a biological surfactant to maintain individual mitotic chromosomes dispersed in the cytoplasm after nuclear envelope disassembly. It is a prognostic marker in breast and prostate cancer. MKI67 is also a potential cell proliferation marker.

Marker of proliferation Ki-67 (MKI67- antigen identified by monoclonal antibody), which is a part of the mitotic chromosome periphery, acts as a biological surfactant to maintain individual mitotic chromosomes dispersed in the cytoplasm after nuclear envelope disassembly. It is a prognostic marker in breast and prostate cancer. MKI67 is also a potential cell proliferation marker. Ki-67 is considered as poor prognostic factors of adenoid cystic carcinoma.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Marker of proliferation Ki-67 (MKI67- antigen identified by monoclonal antibody) consists of a phosphopeptide-binding forkhead-associated (FHA) domain and a protein phosphatase 1 (PP1)-binding site at the amino- terminal, 16 tandem repeats at the central region and leucine and arginine (LR) pairs at the carboxy- terminal. It is expressed at all active phases (G1, S, G2, and mitosis) of the cell cycle. The gene encoding this protein is localized on human chromosome 10.

Marker of proliferation Ki-67 (MKI67) consists of a phosphopeptide-binding forkhead-associated (FHA) domain and a protein phosphatase 1 (PP1)-binding site at the amino- terminal, 16 tandem repeats at the central region and leucine and arginine (LR) pairs at the carboxy- terminal. It is expressed at all active phases of the cell cycle. The gene encoding this protein is localized on human chromosome 10.

Immunogen

HeLa cell nuclear preparation.

Specificity

Reacts with the Ki-67 nuclear antigen (345 and 395 kDa, and several lower m.w. proteins) in immunoblotting. The product shows an immunohistochemical staining pattern similar to that obtained with other anti-Ki-67/MIB-1 reagents. It labels proliferating cells in frozen human tissue and in formalin-fixed, paraffin-embedded tissue. A microwave heating retrieval step is required for successful labeling of routinely formalin-fixed, paraffin-embedded human tissue.