Analysis Note

Positive ControlHCT116 cells for p300, SW480 cells for truncated APC (p147)

Application

Immunoblotting (1 µg/ml, see comments)

General description

Protein G purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with SP40 cells. Recognizes the ~300 kDa APC protein as well as a variety of truncated forms.

Recognizes full length APC (p300) in HCT116 cells and truncated APC (p147) in SW480 cells.Antibody Target Gene Symbol: APC Target Synonym: AI047805, Apc7, AU020952, AW124434, BTPS2, DP2, DP2.5, DP3, Familial adenomatous polyposis, FAP, GS, Min, RATAPC Entrez Gene Name: adenomatous polyposis coli Hu Entrez ID: 324 (Related Antibodies: OP80, ST1150, OP62, OP47L) Mu Entrez ID: 11789 Rat Entrez ID: 24205

Anti-APC (Ab-1), mouse monoclonal, clone FE9, recognizes full length APC (p300) in HCT116 cells and truncated APC (p147) in SW480 cells. It is validated for Western botting.

Immunogen

a synthetic peptide corresponding to the N-terminal 35 amino acids of APC

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Koetsier, P. A., et al. 1993. BioTechniques15, 258.Smith, K. J., et al. 1993. Proc. Natl. Acad. Sci., USA90, 2846.Su, L.-K., et al. 1993. Can. Res.53, 2728.Boynton, R. F., et al. 1992. Proc. Natl. Acad. Sci. USA89, 3385.D′Amico, D., et al. 1992. Cancer Res.52, 1996.Fearon, E. R., and Jones, P. A., 1992. FASEB J.6, 2783.Miyoshi, Y., et al. 1992. Proc. Natl. Acad. Sci. USA89, 4452.Powell, S. M., et al. 1992. Nature359, 235.Groden, J., et al. 1991. Cell66, 589.Kinzler, K. W., et al. 1991. Science253, 661.Nishisho, I., et al. 1991. Science253, 665.

Packaging

100 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In 50 mM sodium phosphate buffer, pH 7.5, 0.2% gelatin.

Warning

Toxicity: Standard Handling (A)