AntiLeukemiaAssociatedRhoGEF/LARGClone13

Application

Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected Leukemia-associated RhoGEF/LARG in HepG2 and HeLa cells.

Anti-Leukemia-associated RhoGEF/LARG Antibody, clone 13D2.1 is a highly specific mouse monoclonal antibody, that targets Leukemia-associated RhoGEF & has been tested in western blotting & ICC.

Research CategoryCell Structure

Research Sub CategoryCytoskeletal Signaling

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The protein named Leukemia-associated RhoGEF (LARG) or alternatively known as Leukemia-associated RhoGEF protein, and encoded by the gene ARHGEF12/KIAA0382/LARG, plays a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13) proteins. Leukemia-associated RhoGEF (LARG) acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase and may act as GTPase-activating protein (GAP) for GNA12 and GNA13. Additionally, Leukemia-associated RhoGEF (LARG) interacts with RHOA, PLXNB1 and PLXNB2, and it interacts with IGF1R beta subunit and GCSAM proteins. Leukemia-associated RhoGEF (LARG) is localized to the cytoplasm and membrane. The protein translocates to the membrane upon stimulation. Leukemia-associated RhoGEF (LARG) widely expressed with isoform specific isoforms being found in certain tissues only, such as isoform 2 is primarily found in the intestine and testis.

Immunogen

GST-tagged recombinant protein corresponding to the RGSL domain of human Leukemia-associated RhoGEF/LARG.

Epitope: RGSL Domain

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in HUVEC cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Leukemia-associated RhoGEF/LARG in 200 µg of HUVEC cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~ 173 kDa observed. Uncharacterized band(s) may appear in some lysates.