Anti-NKCC1/2; clone T4

Application

Research CategorySignaling

This mouse monoclonal Anti-NKCC1/2 Antibody, clone T4, Cat. No. MABS1237 detects levels of NKCC1 & NKCC2, and has been published and validated for use in Electron Microscopy, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, and Western Blotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Solute carrier family 12 member 1 (UniProt Q13621; a.k.a. Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2) and solute carrier family 12 member 2 (UniProt P55011; a.k.a. Basolateral Na-K-Cl symporter, Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1) are encoded by the SLC12A1 (a.k.a. NKCC2; Gene ID 6557) and SLC12A2 (a.k.a. NKCC1; Gene ID 6558) gene, respectively, in human. NKCC1 (SLC12A2) and NKCC2 (SLC12A1) belong to the subfamily of cation-chloride cotransporters (CCCs) that mediate electroneutral transport of sodium, potassium and chloride across the plasma membrane and are inhibited by bumetanide and furosemide-type of compounds. NKCCs play an important role in cell volume regulation, where NKCC2 is involved in reabsorption of salt and osmotically-obliged water in renal epithelial cells, while NKCC1 has a major impact on intracellular chloride ion concentration ([Cl-]i) in vascular smooth muscle cells (VSMCs) and neuronal cells. In primary hypertension, NKCC1 activity is increased in VSMCs and paraventricular nucleus (PVN) neurons, leading to elevated peripheral resistance in systemic circulation and activation of sympathetic nerve system (SNS), respectively. Human NKCC1 and NKCC2 are 12-transmembrane proteins having their N- and C-terminal ends exposed at the cytoplasmic side (a.a. 1-285 and 921-1212 of NKCC1; a.a. 1-177 and 814-1099 of NKCC2).

Immunogen

Recombinant human NKCC1 C-terminal fragment.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4) 150 mM NaCl with 0.05% sodium azide

Protein G purified.

Format: Purified

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected NKCC in 10 µg of HeLa cell lysate.

Specificity

Clone T4 recognizes both NKCC1 and NKCC2 by targeting a C-terminal region epitope exposed only under denaturing condition, and unaccessible for antibody binding when NKCC assumes its native conformation (Lytle, C., et al. (1995). Am. J. Physiol. 269(6 Pt 1):C1469-C1505).

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~165 kDa observed. Target bands appear larger than the calculated molecular weights of 131.4/129.7 kDa (human NKCC1 isoform 1/2), 121.5/121.4 kDa (human NKCC2 isoform A/F), 131.0 kDa (mouse NKCC1), 120.4/120.3/120.3 kDa (mouse NKCC2 isoform A/B/F), and 130.4/120.6 kDa (rat NKCC1/NKCC2) due to glycosylation. Uncharacterized bands may be observed in some lysate(s).